尊龙凯时

北京尊龙凯时最新引进奥地利BIOMEDICA公司生产的可溶性总神经纤毛蛋白-1检测试剂盒(酶联免疫法)，欢迎广大科研用户前来咨询。

可溶性总神经纤毛蛋白-1检测试剂盒(酶联免疫法)

Total Soluble Neuropilin-1 ELISA

全球首家推出由表位定位特异性抗体定量检测

nNrp-1在神经系统中与其配体 semaphorins (信号素）结合而在轴突中起作用， Nrp-1
， Nrp-2为 Sema受体并且仅与III型Sema结合。

应用领域
：骨学，肿瘤学，肾脏学，心血管
。

试剂盒信息

试剂盒信息

货号	BI-20409
方法学	夹心 ELISA, HRP/TMB, 12x8人份测试
样本类型	血清 、血浆(EDTA, 肝素,枸橼酸钠)、细胞培养液
标准品范围	0-12 nmol/l (7个标准品）
标准品	0/0.37/0.75/1.5/3/6/12 nmol/l
质控品	2个
样本量	10 μl /测试
孵育时间	30 min/2h/1h/30min
单位换算	1ng/ml = 0.014nmol/l (MW: 69.7 kDa)
灵敏度	LOD: 0.09 nmol/l (0 pmol/l + 3 SD); LLOQ: 0.09nmol/l
精密度	批内差 (n=6) ≤ 11%, 批间差 (n=12) ≤ 10%
健康人群参考范围	Median 血清( n=24): 2.0nmol/l.   Median EDTA 血浆(n=24): 1.7nmol/l.   Median 肝素 血浆(n=24): 1.7nmol/l.   Median枸橼酸钠血浆(n=24): 1.9nmol/l.   推荐建立实验室自己的参考范围。
非人属种Nrp-1	猴子，猪 ，大鼠及小鼠与人Nrp-1同源性超过90%，本试剂并未对非人属种样本做验证，但是大鼠及小鼠的加标回收率以及线性数据表现合格 。

参考文献

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2.Neuropilin signalling in angiogenesis. Koch S, Biochem Soc Trans, 2012; 40(1):20-25. 

3. Characterization of neuropilin-1 structural features that confer binding to semaphorin 3A and vascular endothelial growth factor 165. Gu C et al., J Biol Chem, 2002; 277(20): 18069-18076.

4. Multifaceted Role of Neuropilins in the Immune System: Potential Targets for Immunotherapy. Roy S et al., Front Immunol, 2017; 10 (8): 1228. 

5.Neuropilin 1 expression in human aortas, coronaries and the main bypass grafts. Alattar M et al., Eur J Cardiothorac Surg, 2014; 46(6): 967-973.

6. Role of Neuropilin-1 in Diabetic Nephropathy. Bondeva T et al., J Clin Med, 2015; 4(6): 1293-1311.

7.Neuropilins: role in signalling, angiogenesis and disease. Zachary I, Chem Immunol Allergy, 2014; 99: 37–70. 

8. Neuropilin-1 is upregulated in the adaptive response of prostate tumors to androgen-targeted therapies and is prognostic of metastatic progression and patient mortality. Tse BWC et al., Oncogene, 2017; 36(24): 3417-3427. 

9. Dual Function of NRP1 in Axon Guidance and Subcellular Target Recognition in Cerebellum. Telley L et al., Neuron, 2016; 21; 91(6): 1276-1291.

10. Nrp1, a Neuronal Regulator, Enhances DDR2-ERK-Runx2 Cascade in Osteoblast Differentiation via Suppression of DDR2 Degradation. Zhang Y et al., Cell Physiol Biochem, 2015; 36(1): 75-84.

11.Neuropilins: structure, function and role in disease. Pellet-Many C et al., Biochem J, 2008; 411(2): 211-226.

12. Neuropilin-1 as Therapeutic Target for Malignant Melanoma. G Graziani1 and PM Lacal, Front Oncol, 2015; 5: 125. 

13. CPMP/ICH/381/95 ICH Topic Q2 (R1) „Validation of Analytical Procedures: Text and Methodology” including: ICH Q2A “Text on Validation of Analytical Procedures” ICH Q2B “Validation of Analytical Procedures: Methodology”.

14.Neuropilins lock secreted semaphorins ontoplexins in a ternary signaling complex. Janssen BJC et al., Nat struct Mol Biol, 2012; 19:1293-1299.