可溶性總神經纖毛蛋白-1檢測試劑盒(酶聯免疫法)
Total Soluble Neuropilin-1 ELISA
全球首家推出由表位定位特異性抗體定量檢測
nNrp-1在神經係統中與其配體 semaphorins (信號素）結合而在軸突中起作用 ， Nrp-1 ， Nrp-2為 Sema受體並且僅與III型Sema結合 。
應用領域 ：骨學 ，腫瘤學 ，腎髒學，心血管 。
試劑盒信息
試劑盒信息
貨號 |
BI-20409 |
方法學 |
夾心 ELISA, HRP/TMB, 12x8人份測試 |
樣本類型 |
血清 、血漿(EDTA, 肝素,枸櫞酸鈉) 、細胞培養液 |
標準品範圍 |
0-12 nmol/l (7個標準品） |
標準品 |
0/0.37/0.75/1.5/3/6/12 nmol/l |
質控品 |
2個 |
樣本量 |
10 μl /測試 |
孵育時間 |
30 min/2h/1h/30min |
單位換算 |
1ng/ml = 0.014nmol/l (MW: 69.7 kDa) |
靈敏度 |
LOD: 0.09 nmol/l (0 pmol/l + 3 SD); LLOQ: 0.09nmol/l |
精密度 |
批內差 (n=6) ≤ 11%, 批間差 (n=12) ≤ 10% |
健康人群參考範圍 |
Median 血清( n=24): 2.0nmol/l. Median EDTA 血漿(n=24): 1.7nmol/l. Median 肝素 血漿(n=24): 1.7nmol/l. Median枸櫞酸鈉血漿(n=24): 1.9nmol/l. |
非人屬種Nrp-1 |
猴子 ，豬 ，大鼠及小鼠與人Nrp-1同源性超過90% ，本試劑並未對非人屬種樣本做驗證 ，但是大鼠及小鼠的加標回收率以及線性數據表現合格 。
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參考文獻
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8. Neuropilin-1 is upregulated in the adaptive response of prostate tumors to androgen-targeted therapies and is prognostic of metastatic progression and patient mortality. Tse BWC et al., Oncogene, 2017; 36(24): 3417-3427.
9. Dual Function of NRP1 in Axon Guidance and Subcellular Target Recognition in Cerebellum. Telley L et al., Neuron, 2016; 21; 91(6): 1276-1291.
10. Nrp1, a Neuronal Regulator, Enhances DDR2-ERK-Runx2 Cascade in Osteoblast Differentiation via Suppression of DDR2 Degradation. Zhang Y et al., Cell Physiol Biochem, 2015; 36(1): 75-84.
11.Neuropilins: structure, function and role in disease. Pellet-Many C et al., Biochem J, 2008; 411(2): 211-226.
12. Neuropilin-1 as Therapeutic Target for Malignant Melanoma. G Graziani1 and PM Lacal, Front Oncol, 2015; 5: 125.
13. CPMP/ICH/381/95 ICH Topic Q2 (R1) „Validation of Analytical Procedures: Text and Methodology” including: ICH Q2A “Text on Validation of Analytical Procedures” ICH Q2B “Validation of Analytical Procedures: Methodology”.
14.Neuropilins lock secreted semaphorins ontoplexins in a ternary signaling complex. Janssen BJC et al., Nat struct Mol Biol, 2012; 19:1293-1299.